A COUPLING HISTOCHEMICAL AZO DYE TEST FOR ALKALINE PHOSPHATASE IN THE KIDNEY
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چکیده
منابع مشابه
Histochemical Localization of Alkaline Phosphatase in The
CHEMICAL investigations have shown that alkaline phosphatase is present in the retina (De Conciliis, 1934; Reis, 1951). In these investigations the whole retina was homogenized and the enzyme estimated in extract. Obviously the estimations of enzyme activity by such a necessarily gross method give no idea of the exact distribution of the enzyme in this, functionally, so finely differentiated ti...
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A method based on the familiar coupling azo dye technique has been devised for the study of enzyme distribution in thick specimens. Its application to the study of esterase distribution in whole tadpoles of Xenopus laevis is described in detail. It can also be used to show the distribution of alkaline phosphatase, both in Xenopus tadpoles and in limbs dissected from chick embryos. It has proved...
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Kidney alkaline phosphatase was purified to homogeneity. It is a glycoprotein of about 172,000 molecular weight. Analyses of the subunit structure by sedimentation equilibrium in 6 M guanidine hydrochloride and by gel electrophoresis in sodium dodecyl sulfate indicate that the alkaline phosphatase is a dimer comprising two very similar or identical subunits of about 87,000 molecular weight. The...
متن کاملThe histochemical demonstration of alkaline phosphatase in unfixed frozen sections.
Although, ideally, enzyme histochemistry should be performed on unfixed sections, so as to avoid potential artifacts inherent in chemical fixation, it is usually considered necessary to fix sections so as to stop the 'soluble' enzymes from becoming lost into the incubation medium. However, provided that suitable frozen sections are used, alkaline phosphatase can be demonstrated fully in unfixed...
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The distribution of non-specific alkaline phosphatase (AP) in normal duodenum, rest small intestine and caecum of chicken has been studied by a catalytic histochemistry method. Embryos and chickens ranging between day 11 prior to and day 60 after hatching were used and the findings of AP reaction were correlated with the age. In the small intestine, during foetal life, the AP activity started t...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1944
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)71989-3